Quantitative analysis of organophosphorus pesticides in freshwater using an optimized firefly luciferase-based coupled bioluminescent assay

• Tipo: Artigo em Revista Científica Internacional
• Data: 2014

Autores

Marques, SM (Autor) Outra; Esteves da Silva, JCGE (Autor) FCUP

Revista

Título: Luminescence

Vol. 29

ISSN: 1522-7235

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 7 Citações

Scopus - 6 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-009-CYV

Abstract (EN): In this paper, a coupled bioluminescent assay, relying on the coupling of the enzymes acetylcholinesterase, S-acetyl-coenzyme A synthetase and firefly luciferase, for the detection and quantitation of organophosphorus pesticides, is presented. Using malathion as a model organophosphorus pesticide, the assay was optimized through statistical experimental design methodology, namely Plackett-Burman and central composite designs. The optimized method requires only 20 mu L of sample. The linear range for the assay was 2.5-15 mu M of malathion, with limits of detection and quantitation of 1.5 and 5.0 mu M, respectively. This simple, fast and robust method allows samples to be analyzed at room temperature and without any pretreatment. Copyright (c) 2013 John Wiley & Sons, Ltd.

Idioma: Inglês

Tipo (Avaliação Docente): Científica