The Evolutionary Portrait of Metazoan NAD Salvage

• Tipo: Artigo em Revista Científica Internacional
• Data: 2013

Autores

Joao Carneiro (Autor) Outra; Sara Duarte Pereira (Autor) Outra; Luisa Azevedo (Autor) FCUP; Filipe F C Castro (Autor) FCUP; Paulo Aguiar (Autor) FCUP; Irina S Moreira (Autor) Outra; Antonio Amorim (Autor) FCUP; Raquel M Silva (Autor) Outra

Revista

Título: PLoS ONE

Vol. 8 Nº 1

Página Final: e64674

ISSN: 1932-6203

Editora: Public Library of Science

Indexação

ISI Web of Knowledge - 8 Citações

Scopus - 9 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

CORDIS: Ciências Naturais > Ciências biológicas > Biologia > Genética ; Ciências Naturais > Ciências biológicas > Biologia > Biologia evolutiva

Outras Informações

ID Authenticus: P-005-0N7

DOI: 10.1371/journal.pone.0064674

Abstract (EN): Nicotinamide Adenine Dinucleotide (NAD) levels are essential for cellular homeostasis and survival. Main sources of intracellular NAD are the salvage pathways from nicotinamide, where Nicotinamide phosphoribosyltransferases (NAMPTs) and Nicotinamidases (PNCs) have a key role. NAMPTs and PNCs are important in aging, infection and disease conditions such as diabetes and cancer. These enzymes have been considered redundant since either one or the other exists in each individual genome. The co-occurrence of NAMPT and PNC was only recently detected in invertebrates though no structural or functional characterization exists for them. Here, using expression and evolutionary analysis combined with homology modeling and protein-ligand docking, we show that both genes are expressed simultaneously in key species of major invertebrate branches and emphasize sequence and structural conservation patterns in metazoan NAMPT and PNC homologues. The results anticipate that NAMPTs and PNCs are simultaneously active, raising the possibility that NAD salvage pathways are not redundant as both are maintained to fulfill the requirement for NAD production in some species.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 11