Multiplex STR genotyping: comparison study, population data and new sequence information

• Tipo: Artigo em Livro de Atas de Conferência Internacional
• Data: 2003

Autores

Alves, C (Autor) Outra; Gusmao, L (Autor) Outra; Pereira, L (Autor) Outra; Amorim, A (Autor) FCUP

Ata de Conferência Internacional

Título: PROGRESS IN FORENSIC GENETICS 9

Páginas: 131-135

19th International Congress of the International-Society-Forensic-Genetics

MUNSTER, GERMANY, AUG 28-SEP 01, 2001

Indexação

ISI Web of Knowledge - 12 Citações

Scopus - 13 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Outras ciências médicas

Outras Informações

ID Authenticus: P-000-KHF

DOI: 10.1016/s0531-5131(02)00623-4

Abstract (EN): A simultaneous study was performed using Perkin-Elmer Profiler Plus (PP)/GenePrint CTTv and Promega Powerplex 16 (P16) kits, which share 12 STR loci, in two population samples from North Portugal (N=110) and Mozambique (N=110). Population data for the three STRs included only in the Powerplex 16 kit (D16S539, Penta D and Penta E) are reported for both populations. No deviations from Hardy-Weinberg equilibrium were observed for all markers in both population samples. A comparative analysis between our data and Promega's (Caucasian-Americans and African-Americans) is also presented. Inconsistencies between genotypes obtained by both kits were detected for D5S818 (3/110 individuals from North Portugal) and D8S1179 (1/110 individuals from Mozambique) and are due to polymorphisms in the primer annealing regions. For D5S818 locus, the inconsistencies are due to a Promega primer annealing failure. In both populations, other point mutations outside the repeat region and structural differences from the consensus repeat motif were also detected. For D8S1179, the observed inconsistency is due to a Perkin-Elmer primer annealing failure.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 5