Characterization of four short tandem repeat loci (THO1, VWA31/A, CD4, and TP53) in northern Portugal

• Tipo: Artigo em Revista Científica Internacional
• Data: 1997

Autores

Amorim, A (Autor) FCUP; Gusmao, L (Autor) Outra; Prata, MJ (Autor) FCUP; Silva, F (Autor) Outra; Bessa, I (Autor) Outra

Revista

Título: Human Biology

Vol. 69

Páginas: 31-40

ISSN: 0018-7143

Editora: Wayne State University Press

Indexação

ISI Web of Knowledge - 22 Citações

Scopus - 20 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-001-C0Z

Abstract (EN): Allele and genotype frequencies for two tetrameric and two pentameric short tandem repeat (STR) loci (THO1, VWA31/A, CD4, and TP53) were determined in a population sample from northern Portugal. Genotyping of PCR amplification products was done using polyacrylamide gel electrophoresis followed by silver staining; heteroduplex analysis was performed to distinguish THO1 genotypes involving allele 10 and the nonconsensus allele 9.3. For all loci allele frequencies fitted the distribution patterns generally observed in European populations, The observed genotype distributions do not deviate significantly from Hardy-Weinberg expectations, although for VWA31/A a significant excess of heterozygotes involving allele 17 was found. Mother-child pair analyses confirmed the regular Mendelian pattern of inheritance, Because the information content of these systems is high and because their genotyping is technically reliable and simple, CD4, THO1, VWA31/A, and TP53 are appropriate genetic systems for anthropological genetics.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10