Detection of Anatoxin-a and Three Analogs in Anabaena spp. Cultures: New Fluorescence Polarization Assay and Toxin Profile by LC-MS/MS

• Tipo: Artigo em Revista Científica Internacional
• Data: 2014

Autores

Jon A Sanchez (Autor) Outra; Paz Otero (Autor) Outra; Amparo Alfonso (Autor) Outra; Vitor Ramos (Autor) Outra; Vitor Vasconcelos (Autor) FCUP; Romulo Araoz (Autor) Outra; Jordi Molgo (Autor) Outra; Mercedes R Vieytes (Autor) Outra; Luis M Botana (Autor) Outra

Revista

Título: Toxins

Vol. 6

Páginas: 402-415

ISSN: 2072-6651

Editora: MDPI

Indexação

ISI Web of Knowledge - 23 Citações

Scopus - 24 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Medicina básica

Outras Informações

ID Authenticus: P-009-3KK

DOI: 10.3390/toxins6020402

Abstract (EN): Anatoxin-a (ATX) is a potent neurotoxin produced by several species of Anabaena spp. Cyanobacteria blooms around the world have been increasing in recent years; therefore, it is urgent to develop sensitive techniques that unequivocally confirm the presence of these toxins in fresh water and cyanobacterial samples. In addition, the identification of different ATX analogues is essential to later determine its toxicity. In this paper we designed a fluorescent polarization (FP) method to detect ATXs in water samples. A nicotinic acetylcholine receptor (nAChR) labeled with a fluorescein derivative was used to develop this assay. Data showed a direct relationship between the amount of toxin in a sample and the changes in the polarization degree of the emitted light by the labeled nAChR, indicating an interaction between the two molecules. This method was used to measure the amount of ATX in three Anabaena spp. cultures. Results indicate that it is a good method to show ATXs presence in algal samples. In order to check the toxin profile of Anabaena cultures a LC-MS/MS method was also developed. Within this new method, ATX-a, retention time (RT) 5 min, and three other molecules with a mass m/z 180.1 eluting at 4.14 min, 5.90 min and 7.14 min with MS/MS spectra characteristic of ATX toxin group not previously identified were detected in the Anabaena spp. cultures. These ATX analogues may have an important role in the toxicity of the sample.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 14