Peptide diversity in strains of the cyanobacterium Microcystis aeruginosa isolated from Portuguese water supplies

• Tipo: Artigo em Revista Científica Internacional
• Data: 2009

Autores

Joana Martins (Autor) Outra; Martin L Saker (Autor) Outra; Cristiana Moreira (Autor) Outra; Martin Welker (Autor) Outra; Jutta Fastner (Autor) Outra; Vitor M Vasconcelos (Autor) FCUP

Revista

Título: Applied Microbiology and Biotechnology

Vol. 82

Páginas: 951-961

ISSN: 0175-7598

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 38 Citações

Scopus - 40 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-003-KY1

DOI: 10.1007/s00253-009-1877-z

Abstract (EN): Strains of the cyanobacterium Microcystis aeruginosa were isolated into pure culture from a variety of lakes, rivers, and reservoirs in Portugal. Samples were tested with matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) to investigate the presence of various peptide groups including aeruginosins, microginins, anabaenopeptins, cyanopeptilins, microcystins, and microviridins and other peptide-like compounds. Binary data, based on the presence and absence of different peptide groups, were analyzed by phylogenetic inference. DNA was also extracted from the samples and tested using a range of primers. Those strains that gave positive results for a Microcystis-specific primer pair were further analyzed for the presence of genes linked to the biosynthesis of microginin and microcystin. The results showed that a wide range of microcystin forms were produced by the strains among which MC-LR, -FR, -RR, -WR, and -YR were the most common. The peptide profiles obtained from the MALDI analysis were assessed using cluster analysis which resulted in the formation of distinct groups or chemotypes.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 11