VIABILITY AND RESPIRATORY ACTIVITY OF PSEUDOMONAS-SYRINGAE CELLS STARVED IN BUFFER

• Tipo: Artigo em Revista Científica Internacional
• Data: 1995

Autores

cabral, jps (Autor) FCUP

Revista

Título: Canadian Journal of Microbiology

Vol. 41

Páginas: 372-377

ISSN: 0008-4166

Editora: NRC Research Press

Indexação

ISI Web of Knowledge - 5 Citações

Scopus - 4 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-001-H2J

Abstract (EN): Pseudomonas syringae cells starved in buffer released orcinol-reactive molecules and materials that absorbed ultraviolet light. The number of cells culturable in nutrient medium decreased more rapidly than the number of intact particles determined by microscopy. The results suggested that starvation resulted in the lysis of an increasing number of cells, and that a fraction of the intact particles were not culturable. Starvation also resulted in a decrease in the rate of oxygen consumption with acetate, glycerol, and succinate, but at different levels. Whereas the respiration of acetate and glycerol decreased concomitantly with culturability, the respiration of succinate decreased to levels similar to the concentration of intact cells, suggesting that all intact particles respired the succinate, but only the culturable cells respired the acetate and glycerol. The results suggest that measuring the activity of the electron-transport system can overestimate the viability of starved bacterial cells, and that complex metabolic activities such as the respiration of acetate and glycerol are probably better suited for the evaluation of this parameter.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6