Dehydroluciferyl-AMP is the main intermediate in the luciferin dependent synthesis of Ap(4)A catalyzed by firefly luciferase

• Tipo: Artigo em Revista Científica Internacional
• Data: 1998

Autores

fontes, r (Autor) FMUP; ortiz, b (Autor) Outra; de diego, a (Autor) Outra; sillero, a (Autor) Outra; sillero, mag (Autor) Outra

Revista

Título: FEBS Letters

Vol. 438

Páginas: 190-194

ISSN: 0014-5793

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 50 Citações

Scopus - 51 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-001-6S1

DOI: 10.1016/s0014-5793(98)01301-5

Abstract (EN): It was previously assumed that E.LH2-AMP was the intermediate complex in the synthesis of Ap(4)A catalyzed by firefly luciferase (EC 1.13.12.7), when luciferin (LH2) was used as cofactor. However, here we show that LH2 is partly transformed, shortly after the onset of the luciferase reaction, to dehydroluciferin (L) with formation of an E.L-AMP complex which is the main intermediate for the synthesis of Ap(4)A. Formation of three more derivatives of LH2 were also observed, related to the production of light by the enzyme, CoA, a known stimulator of light production, inhibits the synthesis of Ap(4)A by reacting with the E.L-AMP complex and yielding L-CoA. (C) 1998 Federation of European Biochemical Societies.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 5