Chemical synthesis and firefly luciferase produced dehydroluciferyl-coenzyme A

• Tipo: Artigo em Revista Científica Internacional
• Data: 2004

Autores

Fraga, H (Autor) Outra; da Silva, JCGE (Autor) FCUP; Fontes, R (Autor) FMUP

Revista

Título: Tetrahedron Letters

Vol. 45

Páginas: 2117-2120

ISSN: 0040-4039

Editora: Elsevier

Indexação

ISI Web of Knowledge - 12 Citações

Scopus - 12 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-000-BJ1

DOI: 10.1016/j.tetlet.2004.01.050

Abstract (EN): Dehydroluciferyl-coenzyme A (L-CoA) was chemically synthesized and characterized by MS, UV-vis spectrometry and RP-HPLC. The identity of the chemically synthesized compound with the one that was produced by firefly luciferase was confirmed. Moreover, the reversibility of the enzymatic conversion of dehydroluciferin reversible arrow dehydroluciferyl-adenylate reversible arrow L-CoA was also confirmed. The chemical synthesis of L-CoA, described here, may help the clarification of the activator effect of CoA on luciferase bioluminescent assays, in which the enzyme catalyzed formation of L-CoA and the consequent destruction of L-AMP is one of the possible explanations for that effect.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 4