Firefly luciferase produces hydrogen peroxide as a coproduct in dehydroluciferyl adenylate formation

• Tipo: Artigo em Revista Científica Internacional
• Data: 2006

Autores

Fraga, H (Autor) Outra; Fernandes, D (Autor) Outra; Novotny, J (Autor) Outra; Fontes, R (Autor) FMUP; da Silva, JCG (Autor) FCUP

Revista

Título: ChemBioChem

Vol. 7

Páginas: 929-935

ISSN: 1439-4227

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 49 Citações

Scopus - 52 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Medicina básica

Outras Informações

ID Authenticus: P-004-KAT

DOI: 10.1002/cbic.200500443

Abstract (EN): Firefly luciferase catalyzes the synthesis of H2O2 from the same substrates as the bioluminescence reaction: ATP and luciferin (D-LH2). About 80% of the enzyme-bound intermediate D-luciferyl adenylate (D-LH2-AMP) is oxidized into oxyluciferin, and a photon is emitted during this reaction. The enzyme pathway responsible for the generation of H2O2 is a side reaction in which D-LH2-AMP is oxidized into dehydroluciferyl adenylate (L-AMP). Like the bioluminescence reaction, the luciferase-catalyzed synthesis of H2O2 and L-AMP is a stereospecific process, involving only the natural D enantiomer. However, the intramolecular electron transfer postulated as essential to the light emission process is not involved in this side reaction.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7