Spatially distinct mitochondrial populations exhibit different mitofilin levels

• Tipo: Artigo em Revista Científica Internacional
• Data: 2012

Autores

ferreira, rmp (Autor) Outra; vitorino, r (Autor) Outra; padrao, ai (Autor) Outra; moreira-goncalves, d (Autor) Outra; alves, rmp (Autor) Outra; duarte, ja (Autor) FADEUP; amado, f (Autor) Outra

Revista

Título: Cell Biochemistry and Function

Vol. 30

Páginas: 395-399

ISSN: 0263-6484

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 7 Citações

Scopus - 7 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-002-8ES

DOI: 10.1002/cbf.2811

Abstract (EN): Subsarcolemmal (SS) and intermyofibrillar (IMF) mitochondria exhibit unique biochemical and functional properties; however, their association with structural membrane proteins that control mitochondrial morphology and functionality in striated muscle tissue was never reported. In IMF and SS mitochondria isolated from rat heart and gastrocnemius muscle, we analysed the expression levels of mitofilin, a mitochondria-associated protein involved in organelle structure maintenance. The statistically significant higher amounts of mitofilin detected in IMF compared with SS mitochondria, 37-fold in cardiac tissue and 3.8-fold in gastrocnemius, together with the specific energetic requirements of these mitochondrial populations highlight the importance of mitofilin in oxidative phosphorylation functionality and in mitochondrial plasticity in striated muscle. The differential expression levels of mitofilin between IMF and SS also suggest that this protein can be used as a specific molecular marker to comparatively discriminate spatially distant mitochondrial populations. Copyright (C) 2012 John Wiley & Sons, Ltd.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 5