iTRAQ-based quantitative proteomic analysis of submandibular glands from rats with STZ-induced hyperglycemia

• Tipo: Artigo em Revista Científica Internacional
• Data: 2013

Autores

alves, rmp (Autor) Outra; vitorino, r (Autor) Outra; padrao, ai (Autor) Outra; moreira-goncalves, d (Autor) Outra; duarte, ja (Autor) FADEUP; ferreira, rmp (Autor) Outra; amado, f (Autor) Outra

Revista

Título: Journal of Biochemistry

Vol. 153

Páginas: 209-220

ISSN: 0021-924X

Editora: Oxford University Press

Indexação

ISI Web of Knowledge - 5 Citações

Scopus - 4 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-002-0ZE

DOI: 10.1093/jb/mvs142

Abstract (EN): The impairment of salivary glands activity is often connected to the complaints of dry-mouth and subsequent degradation of the periodontium of diabetic patients. In this context, submandibular glands (SMGs) play a central role in saliva production and so the understanding of the molecular pathways affected is of paramount importance. Using a streptozotocin-induced hyperglycemia rat model and two different time points (2 and 4 months), we applied mass spectrometry-based proteomic techniques, validated with standard western blot analysis, to identify and quantify the effect of chronic hyperglycemia on the proteome of SMGs. We observed significant variations of proteins such as kallikreins, protein S100A6 or annexins. After 2 months of hyperglycemia, we observed an early phase response characterized by a significant increase of protein S100A6, linked to the inflammatory response, together with the impairment of metabolic and energy production processes. On the other hand, vesicular transport appeared to be favoured in such conditions. Interestingly, in a long-term response to hyperglycemia after 4 months of exposure, we observed a general attenuation of the variations. In conclusion, we present data that support the existence of an adaptation of the gland to long-term stress.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 12