Endurance training limits the functional alterations of heart rat mitochondria submitted to in vitro anoxia-reoxygenation

• Tipo: Artigo em Revista Científica Internacional
• Data: 2006

Autores

Ascensao, A (Autor) FADEUP; Magalhaes, J (Autor) FADEUP; Soares, JMC (Autor) Outra; Ferreira, R (Autor) Outra; Neuparth, MJ (Autor) Outra; Marques, F (Autor) FFUP; Oliveira, PJ (Autor) Outra; Duarte, JA (Autor) FADEUP

Revista

Título: International Journal of Cardiology

Vol. 109 Nº 2

Páginas: 169-178

ISSN: 0167-5273

Editora: Elsevier

Indexação

ISI Web of Knowledge - 36 Citações

Scopus - 40 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Medicina clínica

Outras Informações

ID Authenticus: P-004-KSQ

DOI: 10.1016/j.ijcard.2005.06.003

Abstract (EN): Background: Studies analysing the effect of endurance training on heart mitochondrial function submitted to in vitro anoxia-reoxygenation (A-R) are missing. The present study aimed to investigate the effect of moderate endurance treadmill training (14 weeks) against rat heart mitochondrial dysfunction induced by in vitro A-R. Methods. Respiratory parameters (state 3, state 4, ADP/O and respiratory control ratio-RCR) and oxidative damage markers (carbonyl groups and malondialdehyde) were determined in isolated mitochondria before and after 1 min anoxia followed by 4 min reoxygenation. Levels of heat shock protein 60 kDa (HSP60) and 70 kDa (HSP70) were measured before A-R in mitochondria and whole muscle homogenate, respectively. Results: A-R significantly impaired the rate of state 3 and state 4 respiration, as well as the RCR and ADP/O in the sedentary group. However, mitochondrial state 3 respiration was significantly higher in trained than in the sedentary group both before and after A-R. The impairments in RCR, ADP/O ratio and state 4 induced by A-R in sedentary group were significantly attenuated in endurance-trained group. The inhibition of state 4 induced by GDP was significantly higher in trained than in sedentary group. Oxidative modifications of mitochondrial proteins and phospholipids were found in sedentary group after A-R, although limited in trained group. Increased levels of mitochondrial HSP60 and tissue HSP70 accompanied the lower decrease in the respiratory function after A-R observed in trained group. Conclusion: We therefore concluded that endurance training limited the impairments on rat heart mitochondria caused by the oxidant insult inflicted by in vitro A-R.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10