Tissue expression of PPAR-alpha isoforms in Scophthalmus maximus and transcriptional response of target genes in the heart after exposure to WY-14643

• Tipo: Artigo em Revista Científica Internacional
• Data: 2013

Autores

urbatzka, r (Autor) Outra; galante-oliveira, s (Autor) Outra; rocha, e (Autor) FCUP; castro, l (Autor) Outra; cunha, i (Autor) Outra

Revista

Título: Fish Physiology and Biochemistry

Vol. 39

Páginas: 1043-1055

ISSN: 0920-1742

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 13 Citações

Scopus - 15 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-006-8XF

DOI: 10.1007/s10695-012-9761-7

Abstract (EN): Peroxisome proliferator-activated receptors (PPARs) are involved in the regulation of lipid and carbohydrate metabolism and can be activated either by natural ligands as fatty acids or by synthetic ligands including several environmental chemicals. In this study, two PPAR alpha isoforms (alpha 1 and alpha 2) were analyzed in turbot (Scophthalmus maximus) for a different tissue distribution. PPAR alpha 1 was ubiquitously expressed, while the PPAR alpha 2 was predominantly expressed in the heart. Following this result, turbot juveniles were exposed by injection to a synthetic selective PPAR alpha agonist, WY-14643, for 14 days. Suppression subtractive hybridization (SSH) was performed with pools of heart samples of control and exposed fish to get insights into PPAR alpha-regulated genes in the heart of juvenile turbot. Four genes were positively identified in the forward-subtracted and 12 genes in the reverse-subtracted cDNA SSH library, corresponding to the down-regulated and up-regulated genes in response to the WY-14643 treatment, respectively. The confirmation of these results in individual samples of juvenile turbot exposed to WY-14643 revealed a statistically significant mRNA induction of two cardiac muscle proteins (myosin light chain 2 and tropomyosin 4), which were shown to be involved in heart contraction and heartbeat regulation in other teleost species. Herewith, we showed for the first time that PPAR alpha 2 is predominantly expressed in the heart and that a PPAR alpha agonist can induce the mRNA expression of cardiac muscle proteins in teleosts.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 13