Expression of MhcCyca class I and class II molecules in the early life history of the common carp (Cyprinus carpio L.)

• Tipo: Artigo em Revista Científica Internacional
• Data: 1998

Autores

Rodrigues, PNS (Autor) ICBAS; Hermsen, TT (Autor) Outra; van Maanen, A (Autor) Outra; Taverne Thiele, AJ (Autor) Outra; Rombout, JHMW (Autor) Outra; Dixon, B (Autor) Outra; Stet, RJM (Autor) Outra

Revista

Título: Developmental and Comparative Immunology

Vol. 22

Páginas: 493-506

ISSN: 0145-305X

Editora: Elsevier

Indexação

ISI Web of Knowledge - 23 Citações

Scopus - 27 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Medicina básica

Outras Informações

ID Authenticus: P-008-QB0

DOI: 10.1016/s0145-305x(97)00059-1

Abstract (EN): In this study transcription of class I proportional to chain (Cyca-UA), beta(2)-microglobulin (Cyca-B2m) and class II proportional to (Cyca-DXA) and beta (Cyca-DAB) during the early stages of embryo development was investigated by semi-quantitative PCR, No transcripts of the genes under investigation were detected in the unfertilized egg. The expression of the genes encoding for the class LI molecules revealed to be synchronized starting at day I, unlike those for the class I molecules. Transcription of Cyca-B2m was first detected at day 7, whereas Cyca-UA was already present on day 1. This discrepancy mould suggest absence of class I molecules during early development, The transcription of the Mite genes in lymphoid organs was well established on day 21, with the exception of the spleen. In later stages of ontogeny cell surface expression of class I molecules was studied using polyclonal antibodies to Cyca-UA and Cyca-B2m in conjunction with detection of surface Tg. In week 3-10 Cyca-B2m was found on a higher percentage of cells from pronephros, spleen and thymus compared to Cyca-UA, suggesting the use of an alternative class I proportional to chain, In the thymus, unlike the other organs, this difference remained present in the adult stage. The most likely candidates are a chains encoded by non-classical class I genes.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 14