Transferrin and ferritin response to bacterial infection: The role of the liver and brain in fish

• Tipo: Artigo em Revista Científica Internacional
• Data: 2009

Autores

Joao V Neves (Autor) Outra; Jonathan M Wilson (Autor) Outra; Pedro N S Rodrigues (Autor) ICBAS

Revista

Título: Developmental and Comparative Immunology

Vol. 33

Páginas: 848-857

ISSN: 0145-305X

Editora: Elsevier

Indexação

ISI Web of Knowledge - 94 Citações

Scopus - 118 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Medicina básica

Outras Informações

ID Authenticus: P-003-PNF

DOI: 10.1016/j.dci.2009.02.001

Abstract (EN): Iron is essential for growth and survival, but it is also toxic when in excess. Thus, there is a tight regulation of iron that is accomplished by the interaction of several genes including the iron transporter transferrin and iron storage protein ferritin. These genes are also known to be involved in response to infection. The aim of this study was to understand the role of transferrin and ferritin in infection and iron metabolism in fish. Thus, sea bass transferrin and ferritin H cDNAs were isolated from liver, cloned and characterized. Transferrin constitutive expression was found to be highest in the liver, but also with significant expression in the brain, particularly in the highly vascularized region connecting the inferior lobe of the hypothalamus and the saccus vasculosus. Ferritin, on the other hand, was expressed in all tested organs, but also significantly higher in the liver. Fish were subjected to either experimental bacterial infection or iron modulation and transferrin and ferritin mRNA expression levels were analyzed, along with several iron regulatory parameters. Transferrin expression was found to decrease in the liver and increase in the brain in response to infection and to increase in the liver in iron deficiency. Ferritin expression was found to inversely reflect transferrin in the liver, increasing in infection and iron overload and decreasing in iron deficiency, whereas in the brain, ferritin expression was also increased in infection. These findings demonstrate the evolutionary conservation of transferrin and ferritin dual functions in vertebrates, being involved in both the immune response and iron metabolism.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10