Physiologic concentrations of bile salts inhibit rat hepatic alkaline phosphatase but not the intestinal isoenzyme

• Tipo: Artigo em Revista Científica Internacional
• Data: 2000

Autores

Martins, MJ (Autor) FMUP; Negrao, MR (Autor) FMDUP; Hipolito Reis, C (Autor) Outra; Azevedo, I (Autor) Outra

Revista

Título: Clinical Biochemistry

Vol. 33

Páginas: 611-617

ISSN: 0009-9120

Editora: Elsevier

Indexação

ISI Web of Knowledge - 27 Citações

Scopus - 14 Citações

Classificação Científica

FOS: Ciências da engenharia e tecnologias > Engenharia médica

Outras Informações

ID Authenticus: P-000-YHX

DOI: 10.1016/s0009-9120(00)00174-0

Abstract (EN): Objective: The effect of bile salts on alkaline phosphatase (EC 3.1.3.1) activity from Wistar rat liver, duodenum, jejunum, and serum was investigated. Design and results: For concentrations higher than 1 mM conjugated bile salts (glycocholate, glycochenodeoxycholate, taurocholate, taurodeoxycholate, and taurochenodeoxycholate) inhibited hepatic ALP but, up to concentrations of 10 mM, had no effect on intestinal ALP. Also cholate, deoxycholate, and chenodeoxycholate, within the same concentration range, did not have any effect on intestinal ALP. ALP inhibition induced by conjugated bile salts was significantly higher in serum of starved rats than in serum of fed animals, what is in good agreement with the known higher proportion of hepatic ALP and lower proportion of intestinal ALP in serum of starved rats. Conclusions: Bile salts can, thus, be used to help discriminating between tissue-nonspecific and intestinal ALP isoenzymes and identifying pathologic conditions where the relative quantities of these isoenzymes are altered in serum. Inhibition of hepatic ALP by physiologic concentrations of bile salts may bear some relation to the bile salts effects on their own enterohepatic circulation and/or biosynthesis. Copyright (C) 2001 The Canadian Society of Clinical Chemists.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7