Advances and perspectives in Leishmania cell based drug-screening procedures

• Tipo: Outra Publicação em Revista Científica Internacional
• Data: 2007

Autores

Sereno, D (Autor) Outra; Cordeiro C da Silva (Autor) FFUP; Mathieu Daude, F (Autor) Outra; Ouaissi, A (Autor) Outra

Revista

Título: Parasitology International

Vol. 56 Nº 1

Páginas: 3-7

ISSN: 1383-5769

Editora: Elsevier

Indexação

ISI Web of Knowledge - 79 Citações

Scopus - 84 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Ciências da saúde

Outras Informações

ID Authenticus: P-004-BN4

DOI: 10.1016/j.parint.2006.09.001

Abstract (EN): Efforts for the development of new therapeutics, essential for the control of leishmaniasis rely mainly on screening of potentially effective compounds in pathogen growth/multiplication assays, both in vitro and in vivo. Screenings designed to closely reflect the situation in vivo are currently labor-intensive and expensive, since they require intracellular antastigotes and animal models. Screenings designed to facilitate rapid testing of a large number of drugs are not performed on the clinically relevant parasite stage, but the promastigotes. The ability to select transgenic Leishmania expressing reporter proteins, such as the green fluorescent protein (GFP) or the luciferase, opened up new possibilities for the development of drug screening tests. In this review we will focus on available methodologies for direct drug screening purposes against the mammalian stage of the parasite, with emphasis on the future developments that could improve sensitivity, reliability, versatility and the throughput of the intracellular model screening.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 5