IMMUNOADSORPTION PROCEDURE AS A POTENTIAL METHOD FOR THE SPECIFIC BETA-2-MICROGLOBULIN REMOVAL FROM PLASMA OF PATIENTS WITH CHRONIC-RENAL-FAILURE

• Tipo: Artigo em Revista Científica Internacional
• Data: 1995

Autores

vallar, l (Autor) Outra; costa, pmp (Autor) ICBAS; teixeira, a (Autor) Outra; pfister, m (Autor) Outra; barrois, r (Autor) Outra; costa, pp (Autor) Outra; rivat, c (Autor) Outra

Revista

Título: Journal of Chromatography - Biomedical Applications

Vol. 664

Páginas: 97-106

ISSN: 0378-4347

Editora: Elsevier B.V.

Indexação

ISI Web of Knowledge - 20 Citações

Scopus - 18 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-001-H9X

DOI: 10.1016/0378-4347(94)00402-q

Abstract (EN): beta(2)-Microglobulin (beta(2)-M), which accumulates in the plasma of patients undergoing long-term dialysis, has been identified as the principal precursor protein of amyloid fibrils in dialysis-related amyloidosis. As no specific treatment for this affection has been yet established, an extracorporeal immunoadsorption procedure appears to be an attractive therapeutic approach to remove beta(2)-M. Several murine monoclonal antibodies to human beta(2)-M were developed and compared as affinity ligands. One of them was selected on the basis of its specificity and adsorption capacity. In order to achieve maximum efficiency in protein removal, different parameters of the procedure were studied and optimized: effect of antibody coupling density, determination of maximum adsorption capacity of the immunoadsorbents and influence of antigen concentration and of flow-rate on antigen capture efficiency. The conditions of regeneration of immunoaffinity sorbents were also investigated to allow their multiple use without loss of adsorption capacity. The results show the validity of the proposed technique in removing beta-M from plasma of patients with chronic renal failure.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10