Unraveling the Uptake Mechanisms of Mannan Nanogel in Bone-Marrow-Derived Macrophages

• Tipo: Artigo em Revista Científica Internacional
• Data: 2012

Autores

Silvia A Ferreira (Autor) Outra; Alexandra Correia (Autor) ICBAS; Pedro Madureira (Autor) Outra; Manuel Vilanova (Autor) ICBAS; Francisco M Gama (Autor) Outra

Revista

Título: Macromolecular Bioscience

Vol. 12

Páginas: 1172-1180

ISSN: 1616-5187

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 6 Citações

Scopus - 6 Citações

Classificação Científica

FOS: Ciências da engenharia e tecnologias > Engenharia dos materiais

CORDIS: Ciências Tecnológicas > Tecnologia > Biotecnologia

Outras Informações

ID Authenticus: P-002-62F

DOI: 10.1002/mabi.201200075

Abstract (EN): The mechanisms associated with the cellular internalization of nanomedicines must be carefully considered when designing drug- and vaccine-delivery systems. The cellular fate and effects of nanomedicines depend to a large extent on the cell uptake routes. A self-assembled mannan nanogel is developed as a vaccination platform for antigen and adjuvant delivery. The mannan nanogel uptake by murine bone-marrow-derived macrophages is found to be time-, concentration-, and energy-dependent, involving mannose-receptor-mediated phagocytosis and clathrin-mediated endocytosis. The nanogel is also visualized in the cytosol suggesting endolysosomal escape. These results indicate that mannan nanogel is a promising versatile carrier for intracellular delivery of vaccines or therapeutic agents.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9