Characterisation of the last Fe-S cluster-binding subunit of Neurospora crassa complex I

• Tipo: Artigo em Revista Científica Internacional
• Data: 1999

Autores

sousa, r (Autor) Outra; barquera, b (Autor) Outra; duarte, m (Autor) Outra; finel, m (Autor) Outra

Revista

Título: Biochimica et Biophysica Acta - Bioenergetics

Vol. 1411

Páginas: 142-146

ISSN: 0005-2728

Editora: Elsevier

Indexação

ISI Web of Knowledge - 7 Citações

Scopus - 7 Citações

Outras Informações

ID Authenticus: P-001-4K5

DOI: 10.1016/S0005-2728(99)00014-6

Abstract (EN): We have cloned cDNAs encoding the last iron-sulphur protein of complex I from Neurospora crassa. The cDNA sequence contains an open reading frame that codes for a precursor polypeptide of 226 amino acid residues with a molecular mass of 24 972 Da. Our results indicate that the mature protein belongs probably to the peripheral arm of complex I and is rather unstable when not assembled into the enzyme. The protein is highly homologous to the PSST subunit of bovine complex I, the most likely candidate to bind iron-sulphur cluster N-2. All the amino acid residues proposed to bind such a cluster are conserved in the fungal protein. (C) 1999 Elsevier Science B.V. All rights reserved.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 5