The Voltammetric Responses of High and Low Molecular Weight DNA on a Variety of Carbon Substrates; Demonstrating the Benefits of Graphitic Surfaces

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

goncalves, lm (Autor) Outra; batchelor-mcauley, c (Autor) Outra; xiong, lhj (Autor) Outra; barros, aa (Autor) FCUP; compton, rg (Autor) Outra

Revista

Título: Electroanalysis

Vol. 23

Páginas: 583-587

ISSN: 1040-0397

Editora: Wiley-Blackwell

Outras Informações

ID Authenticus: P-002-TPD

DOI: 10.1002/elan.201000637

Abstract (EN): This work compares the voltammetric response of high (similar to 2000 bp) and low (<= 300 bp) molecular weight DNA on a variety of carbon based electrodes demonstrating for both species the advantages of using graphitic electrodes. Results suggest that the edge plane defects are kinetically dominant for the electro-oxidation of nucleobases, whereas the basal plane sites are favoured for adsorption of the DNA to the electrode surface. For the high molecular weight DNA it is shown that although larger currents are obtained through the use of the edge plane electrode, it is beneficial in terms of signal-to-noise ratio to use a basal plane electrode.

Idioma: Inglês

Tipo (Avaliação Docente): Científica