Dynamics of Protein Phosphatase Gene Expression in Corbicula fluminea Exposed to Microcystin-LR and to Toxic Microcystis aeruginosa Cells

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

Jose Carlos Martins (Autor) Outra; Joao Machado (Autor) Outra; Antonio Martins (Autor) Outra; Joana Azevedo (Autor) Outra; Luis OlivaTeles (Autor) FCUP; Vitor Vasconcelos (Autor) FCUP

Revista

Título: International Journal Of Molecular Sciences

Vol. 12

Páginas: 9172-9188

ISSN: 1661-6596

Editora: MDPI

Indexação

ISI Web of Knowledge - 12 Citações

Scopus - 15 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-002-J0S

DOI: 10.3390/ijms12129172

Abstract (EN): This study investigated the in vivo effects of microcystins on gene expression of several phosphoprotein phosphatases (PPP) in the freshwater clam Corbicula fluminea with two different exposure scenarios. Clams were exposed for 96 h to 5 mu g L-1 of dissolved microcystin-LR and the relative changes of gene expression of three different types of PPP (PPP1, 2 and 4) were analyzed by quantitative real-time PCR. The results showed a significant induction of PPP2 gene expression in the visceral mass. In contrast, the cyanotoxin did not cause any significant changes on PPP1 and PPP4 gene expression. Based on these results, we studied alterations in transcriptional patterns in parallel with enzymatic activity of C. fluminea for PPP2, induced by a Microcystis aeruginosa toxic strain (1 x 10(5) cells cm(-3)) during 96 h. The relative changes of gene expression and enzyme activity in visceral mass were analyzed by quantitative real-time PCR and colorimetric assays respectively. The clams exhibited a significant reduction of PPP2 activity with a concomitant enhancement of gene expression. Considering all the results we can conclude that the exposure to an ecologically relevant concentration of pure or intracellular microcystins (-LR) promoted an in vivo effect on PPP2 gene expression in C. fluminea.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 17