Application of real-time PCR in the assessment of the toxic cyanobacterium Cylindrospermopsis raciborskii abundance and toxicological potential

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

Cristiana Moreira (Autor) Outra; Antonio Martins (Autor) Outra; Joana Azevedo (Autor) Outra; Marisa Freitas (Autor) Outra; Ana Regueiras (Autor) Outra; Micaela Vale (Autor) Outra; Agostinho Antunes (Autor) FCUP; Vitor Vasconcelos (Autor) FCUP

Revista

Título: Applied Microbiology and Biotechnology

Vol. 92

Páginas: 189-197

ISSN: 0175-7598

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 27 Citações

Scopus - 28 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-002-MAX

DOI: 10.1007/s00253-011-3360-x

Abstract (EN): Cyanobacteria are prokaryotic photosynthetic microorganisms that pose a serious threat to aquatic environments because they are able to form blooms under eutrophic conditions and produce toxins. Cylindrospermopsis raciborskii is a planktonic heterocystous filamentous cyanobacterium initially assigned to the tropics but currently being found in more temperate regions such as Portugal, the southernmost record for this species in Europe. Cylindrospermopsin originally isolated from C. raciborskii is a cytotoxic alkaloid that affects the liver, kidney, and other organs. It has a great environmental impact associated with cattle mortality and human morbidity. Aiming in monitoring this cyanobacterium and its related toxin, a shallow pond located in the littoral center of Portugal, Vela Lake, used for agriculture and recreational purposes was monitored for a 2-year period. To accomplish this, we used the real-time PCR methodology in field samples to quantify the variation of specific genetic markers with primers previously described characterizing total cyanobacteria (16S rRNA), C. raciborskii (rpoC1), and cylindrospermopsin synthetase gene (pks). The results report the high abundance of both cyanobacteria and C. raciborskii in Vela Lake, with C. raciborskii representing 0.4% to 58% of the total cyanobacteria population. Cylindrospermopsin synthetase gene was detected in one of the samples. We believe that with the approach developed in this study, it will be possible to monitor C. raciborskii population dynamics and seasonal variation, as well as the potential toxin production in other aquatic environments.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9