Diversity of cyanobacterial hydrogenases, a molecular approach

• Tipo: Artigo em Revista Científica Internacional
• Data: 2000

Autores

Tamagnini, P (Autor) FCUP; Costa, JL (Autor) Outra; Almeida, L (Autor) Outra; Oliveira, MJ (Autor) Outra; Salema, R (Autor) Outra; Lindblad, P (Autor) Outra

Revista

Título: Current Microbiology

Vol. 40

Páginas: 356-361

ISSN: 0343-8651

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 61 Citações

Scopus - 70 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-000-ZT7

DOI: 10.1007/s002840010070

Abstract (EN): In an effort to elucidate the diversity of cyanobacterial hydrogenases, we used a molecular approach. Filamentous strains from a broad range of sources were screened for the presence of hup (uptake hydrogenase), xisC (rearrangement within hupL), and hox (bidirectional hydrogenase) genes. As expected, an uptake hydrogenase seems to be present in all N-2-fixing cyanobacteria. On the other hand, no evidence was found for the presence of a conventional bidirectional enzyme in several strains. Similarly, the presence of xisC is not a characteristic shared by all the heterocyst-forming cyanobacteria. Although tempting, it is not possible to establish a correlation between the presence/absence of the bidirectional hydrogenase and the occurrence of xisC. Thr natural molecular variation of hydrogenases in cyanobacteria is certainly a field to explore, both to understand the physiological functions of the respective enzymes and to identify a genetic background to be used when constructing a strain for photobiological H-2 production in a bioreactor.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6