iTRAQ-based quantitative proteomic analysis of Gloeothece sp PCC 6909: Comparison with its sheathless mutant and adaptations to nitrate deficiency and sulfur limitation

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

Sara B Pereira (Autor) Outra; Saw Yen Ow (Autor) Outra; Martin E Barrios Llerena (Autor) Outra; Phillip C Wright (Autor) Outra; Pedro Moradas Ferreira (Autor) ICBAS; Paula Tamagnini (Autor) FCUP

Revista

Título: Journal of Proteomics

Vol. 75

Páginas: 270-283

ISSN: 1874-3919

Editora: Elsevier

Indexação

ISI Web of Knowledge - 10 Citações

Scopus - 12 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-002-HRP

DOI: 10.1016/j.jprot.2011.09.007

Abstract (EN): Gloeothece sp. PCC 6909 is a unicellular N(2)-fixing cyanobacterium with a well defined and highly developed sheath surrounding its cells. A sheathless mutant of this strain was previously obtained by chemical mutagenesis and, although lacking the sheath, it releases large amounts of polysaccharides into the culture medium. To provide a global understanding on the metabolic differences between the two phenotypes, the proteomes of the wild type and mutant were analyzed using a cross-species proteomics approach coupled with iTRAQ isobaric tagging technology, since their genome sequences are not yet available. Effects arising from the presence/absence of nitrate and sulfur are presented as two metabolically directed follow-up iTRAQ studies. These nutrients are believed to play a major role in Gloeothece's metabolism, including the production of extracellular polymeric substances - EPS. 454, 124, and 53 proteins were identified and reliably quantified using homology anchoring approaches for iTRAQ previously described. The results obtained strongly suggest that the chemical mutagenesis affected the regulation of a number of key cellular processes, as revealed by the significant fold changes observed for proteins covering a large spectrum of functional groups. Moreover, they provide new insights on the adaptations of Gloeothece cells to nitrate-deficiency and sulfur-limitation.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 14