Zinc activates neutrophils' oxidative burst

• Tipo: Artigo em Revista Científica Internacional
• Data: 2010

Autores

Marisa Freitas (Autor) Outra; Graca Porto (Autor) ICBAS; Jose L F C Lima (Autor) FFUP; Eduarda Fernandes (Autor) FFUP

Revista

Título: Biometals

Vol. 23 Nº 1

Páginas: 31-41

ISSN: 0966-0844

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 13 Citações

Scopus - 15 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-003-98E

DOI: 10.1007/s10534-009-9264-x

Abstract (EN): Zinc has been shown to disturb the innate host defense response by interfering in the activation of neutrophils and subsequent oxidative burst, although the exact role of this metal, either as an activator or inhibitor, remains a matter of controversy among research groups. These apparent discrepancies may be due to experimental settings, through modification of zinc availability to neutrophils, or to inaccurate detections of reactive species. Thus, the main objective of the present study was to provide clarification on the role of zinc on the activation of human neutrophils and the subsequent oxidative burst. For that purpose, different detection methods and incubation media were used. The obtained results showed that phosphate buffers (PBS and HBSS) complex with zinc and interfere with the results obtained with this metal. By using Tris-G, it was clearly demonstrated that zinc, at low concentrations (5-12.5 mu M), activates NADPH oxidase, mainly via protein kinase C, leading to the formation of superoxide radical (O(2) (aEuro cent a')). Higher concentrations of zinc results on a rapid dismutation of O(2) (aEuro cent a') to oxygen and hydrogen peroxide, which in turn is used by myeloperoxidase to generate hypochlorous acid (HOCl).

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 11