Antioxidant activity of Hypericum androsaemum infusion: Scavenging activity against superoxide radical, hydroxyl radical and hypochlorous acid

• Tipo: Artigo em Revista Científica Internacional
• Data: 2002

Autores

Valentao, P (Autor) FFUP; Fernandes, E (Autor) FFUP; Carvalho, F (Autor) FFUP; Andrade, PB (Autor) FFUP; Seabra, RM (Autor) Outra; Bastos, MD (Autor) FFUP

Revista

Título: Biological and Pharmaceutical Bulletin

Vol. 25

Páginas: 1320-1323

ISSN: 0918-6158

Editora: Pharmaceutical Society of Japan

Indexação

ISI Web of Knowledge - 80 Citações

Scopus - 93 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Medicina básica

Outras Informações

ID Authenticus: P-000-MRS

DOI: 10.1248/bpb.25.1320

Abstract (EN): Hypericum androsaemum is a medicinal plant species containing many polyphenolic compounds, namely flavonoids and phenolic acids. Since polyphenolic compounds have high antioxidant potential, the ability of H. androsaemum infusion to act as a scavenger of reactive oxygen species (superoxide radical, hydroxyl radical and hypochlorous acid) was investigated. Superoxide radical was generated by the xanthine/xanthine oxidase and phenazine methosulphate/NADH systems. The infusion-mediated prevention of nitroblue tetrazolium reduction by the superoxide radical was used as the measured endpoint. Hydroxyl radical was generated by the Fe3+- EDTA/ascorbate Fenton system, and assayed by evaluating deoxyribose degradation using the thiobarbituric acid method. Hypochlorous acid scavenging activity was tested by measuring the inhibition of hypochlorous acid-induced 5-thio-2-nitrobenzoic acid oxidation to 5,5-dithiobis(2-nitrobenzoic acid). The tested infusion mainly exhibited a potent scavenging effect on superoxide radicals (although a noncompetitive inhibitory effect on xanthine oxidase was also observed). The infusion also acted as a moderate scavenger of hydroxyl radicals and hypochlorous acid. A phytochemical study of the infusion was also undertaken, and nine phenolic compounds were identified.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 4