Protective activity of Hypericum androsaemum infusion against tert-butyl hydroperoxide-induced oxidative damage in isolated rat hepatocytes

• Tipo: Artigo em Revista Científica Internacional
• Data: 2004

Autores

Valentao, P (Autor) FFUP; Carvalho, M (Autor) Outra; Fernandes, E (Autor) FFUP; Carvalho, F (Autor) FFUP; Andrade, PB (Autor) FFUP; Seabra, RM (Autor) Outra; Bastos, MD (Autor) FFUP

Revista

Título: Journal of Ethnopharmacology

Vol. 92

Páginas: 79-84

ISSN: 0378-8741

Editora: Elsevier

Indexação

ISI Web of Knowledge - 16 Citações

Scopus - 18 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Medicina básica

Outras Informações

ID Authenticus: P-000-AK3

DOI: 10.1016/j.jep.2004.02.004

Abstract (EN): Hypericum androsaemum L. (Guttiferae) is a medicinal plant with antioxidant activity. Increasing evidence regarding free radical generating agents suggests that hepatotoxic-related disorders may involve reactive oxygen species (ROS). The purpose of this study was to investigate the protective effect of Hypericum androsaemum infusion on isolated rat hepatocytes oxidative injury induced by tert-butyl hydroperoxide (t-BHP). The results showed that pretreatment of the cells with this infusion (16, 62 and 250 mug/ml) prevented the leakage of lactate dehydrogenase (LDH) and lipid peroxidation caused by a 30-min treatment with t-BHP (1 mM). However, infusion-induced alterations on glutathione homeostasis were noticed, as it was observed by the increase in glutathione oxidised form (GSSG) and depletion in total glutathione levels, which indicates that plant-derived antioxidant extracts may not be considered a generalised way of treating pro-oxidant-related diseases. sigma 2004 Elsevier Ireland Ltd. All rights reserved.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6