Simultaneous determination of retinol, beta-carotene and alpha-tocopherol in adipose tissue by high-performance liquid chromatography

• Tipo: Artigo em Revista Científica Internacional
• Data: 2001

Autores

Casal, S (Autor) FFUP; Macedo, B (Autor) Outra; Oliveira, MBPP (Autor) FFUP

Revista

Título: Journal of Chromatography - Biomedical Applications

Vol. 763

Páginas: 1-8

ISSN: 0378-4347

Editora: Elsevier B.V.

Indexação

ISI Web of Knowledge - 38 Citações

Scopus - 41 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-000-SSD

DOI: 10.1016/s0378-4347(01)00349-8

Abstract (EN): A method is described for evaluation of fat-soluble vitamin in human adipose tissue with the aim to obtain, accurately and within the shortest analysis time, a time-integrated measure of exposure to vitamins from the diet. Fat tissue was deproteinized with ethanol and extracted with n-hexane. Normal-phase HPLC was performed in a Lichrosorb Si60 column with a gradient of n-hexane-2-propanol at 1 ml/min. Detection was accomplished using a diode-array system (for retinol and beta -carotene) in series with a fluorescence detector (alpha -tocopherol). The method was validated and applied to human adipose tissue in a total of 140 subjects. The mean contents found were 0.43, 0.84, 240.3 mug/g for retinol, beta -carotene and alpha -tocopherol, respectively. The method is sensitive enough for detecting the compounds in 1.6 mg of adipose tissue considering the lowest concentration found.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8