GC Determination of Acetone, Acetaldehyde, Ethanol, and Methanol in Biological Matrices and Cell Culture

• Tipo: Artigo em Revista Científica Internacional
• Data: 2009

Autores

Helena Pontes (Autor) Outra; de Pinho, PG (Autor) Outra; Susana Casal (Autor) FFUP; Helena Carmo (Autor) FFUP; Agostinho Santos (Autor) Outra; Teresa Magalhaes (Autor) Outra; Fernando Remiao (Autor) FFUP; Felix Carvalho (Autor) FFUP; Maria Lourdes Bastos (Autor) FFUP

Revista

Título: Journal of Chromatographic Science

Vol. 47 Nº 2

Páginas: 272-278

ISSN: 0021-9665

Editora: Oxford University Press

Indexação

ISI Web of Knowledge - 57 Citações

Scopus - 61 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-003-M5K

DOI: 10.1093/chromsci/47.4.272

Abstract (EN): A gas chromatography with flame ionization detection method (GC-FID) with direct injection, using a capillary column, was validated to determine ethanol, acetaldehyde, methanol, and acetone in different human matrices, such as whole blood, vitreous humour, and urine, with clinical and forensic interest. This method was also employed to quantify these compounds in cell culture medium, thus being useful in basic research. A good peak resolution was achieved, with linear correlation between concentration and peak areas for all the compounds in all the matrices. The inter- and intra-day precisions of the method were always under 15% and 10%, respectively. The accuracy of the method, calculated as the percentage of the target concentration, was within the acceptable limits. The obtained limits of detection were below 0.85 mg/L for acetaldehyde and below 0.75 mg/L for the other considered compounds. The small injection volume and the high split ratios applied, allied to the high performance of the GC column, resulted in very good peak resolution and high sensitivities. This method is easy to perform, making it suitable for the routine of clinical biochemistry and forensic laboratories.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7