Molecular determinants of ligand specificity in family 11 carbohydrate binding modules - an NMR, X-ray crystallography and computational chemistry approach

• Tipo: Artigo em Revista Científica Internacional
• Data: 2008

Autores

Aldino Viegas (Autor) Outra; Natercia F Bras (Autor) Outra; Nuno M F S A Cerqueira (Autor) FCUP; Pedro Alexandrino Fernandes (Autor) FCUP; Jose A M Prates (Autor) Outra; Carlos M G A Fontes (Autor) Outra; Marta Bruix (Autor) Outra; Maria Joao Romao (Autor) Outra; Ana Luisa Carvalho (Autor) Outra; Maria Joao Ramos (Autor) FCUP; Anjos L Macedo (Autor) Outra; Eurico J Cabrita (Autor) Outra

Revista

Título: FEBS Journal

Vol. 275

Páginas: 2524-2535

ISSN: 1742-464X

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 29 Citações

Scopus - 29 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-003-ZNE

DOI: 10.1111/j.1742-4658.2008.06401.x

Abstract (EN): The direct conversion of plant cell wall polysaccharides into soluble sugars is one of the most important reactions on earth, and is performed by certain microorganisms such as Clostridium thermocellum (Ct). These organisms produce extracellular multi-subunit complexes (i.e. cellulosomes) comprising a consortium of enzymes, which contain noncatalytic carbohydrate-binding modules (CBM) that increase the activity of the catalytic module. In the present study, we describe a combined approach by X-ray crystallography, NMR and computational chemistry that aimed to gain further insight into the binding mode of different carbohydrates (cellobiose, cellotetraose and cellohexaose) to the binding pocket of the family 11 CBM. The crystal structure of C. thermocellum CBM11 has been resolved to 1.98 angstrom in the apo form. Since the structure with a bound substrate could not be obtained, computational studies with cellobiose, cellotetraose and cellohexaose were carried out to determine the molecular recognition of glucose polymers by CtCBM11. These studies revealed a specificity area at the CtCBM11 binding cleft, which is lined with several aspartate residues. In addition, a cluster of aromatic residues was found to be important for guiding and packing of the polysaccharide. The binding cleft of CtCBM11 interacts more strongly with the central glucose units of cellotetraose and cellohexaose, mainly through interactions with the sugar units at positions 2 and 6. This model of binding is supported by saturation transfer difference NMR experiments and linebroadening NMR studies.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 12