Electrospray tandem mass spectrometry of aminochromes

• Tipo: Artigo em Revista Científica Internacional
• Data: 2001

Autores

Lemos Amado, F (Autor) Outra; Domingues, P (Autor) Outra; Ferrer Correia, A (Autor) Outra; Remiao, F (Autor) FFUP; Milhazes, N (Autor) Outra; Borges, F (Autor) FFUP; Carvalho, FD (Autor) FFUP; Bastos, ML (Autor) FFUP

Revista

Título: Rapid Communications in Mass Spectrometry

Vol. 15

Páginas: 2466-2471

ISSN: 0951-4198

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 16 Citações

Scopus - 15 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-000-XV0

DOI: 10.1002/rcm.498

Abstract (EN): The catecholamines adrenaline, noradrenaline, dopamine, dopa and isoprenaline were oxidized into their respective aminochromes: adrenochrome, noradrenochrome, dopaminochrome, dopachrome and isoprenochrome. Tandem mass spectrometry (MS/MS) fragmentation patterns were examined for the five aminochromes in order to establish a general structural assignment of these oxidation products by electrospray mass spectrometry. Although protonated aminochromes undergo similar fragmentation patterns with a characteristic consecutive loss of two carbonyl groups, the presence of different substituents in the parent compounds led to significant changes in the CID spectra. This feature is more evident for isoprenochrome and dopachrome, especially for the latter where the MS/MS spectrum is dominated by the loss of formic acid. A general pattern of fragmentation for aminochromes is proposed, which should provide a suitable basis to aid their characterization in studies in vivo or in vitro. Copyright (C) 2001 John Wiley & Sons, Ltd.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6