Molecular and genotypic characterization of human thyroid follicular cell carcinoma-derived cell lines

• Tipo: Artigo em Revista Científica Internacional
• Data: 2007

Autores

Ana Margarida Meireles (Autor) Outra; Ana Preto (Autor) Outra; Ana Sofia Rocha (Autor) Outra; Ana Paula Rebocho (Autor) Outra; Valdemar Maximo (Autor) FMUP; Isabel Pereira Castro (Autor) Outra; Severina Moreira (Autor) Outra; Talia Feijao (Autor) Outra; Tiago Botelho (Autor) Outra; Ricardo Marques (Autor) Outra; Vitor Trovisco (Autor) Outra; Luis Cirnes (Autor) Outra; Cintia Alves (Autor) Outra; Sergia Velho (Autor) Outra; Paula Soares (Autor) FMUP; Manuel Sobrinho Simoes (Autor) FMUP

Revista

Título: Thyroid

Vol. 17 Nº 8

Páginas: 707-715

ISSN: 1050-7256

Editora: Mary Ann Liebert

Indexação

ISI Web of Knowledge - 83 Citações

Scopus - 84 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Medicina clínica

Outras Informações

ID Authenticus: P-004-8PQ

DOI: 10.1089/thy.2007.0097

Abstract (EN): Objective: Our aim was to characterize the molecular and genotypic profile of eight thyroid carcinoma-derived cell lines-TPC1, FB2, B-CPAP, K1, XTC-1, C643, 8505C, and Hth74-in order to use them as in vitro models of thyroid carcinogenesis. Design: We evaluated the expression of five thyroid-specific genes (Tg, TSHr, TPO, PAX8, and TTF-1) to establish the cell lineage and to assess the differentiation status of each of the cell lines. We screened for mutations in the most relevant oncogenes/tumor suppressor genes affected in thyroid carcinogenesis: RAS, BRAF, CTNNB1, and TP53 along with RET/PTC rearrangements. Considering the putative relevance in general carcinogenesis, we have also studied other molecules such as EGFR, PI3K, RAF-1, and THRB. To determine the genetic identity of the cell lines, we performed genotypic analysis. Main outcome: The panel of cell lines we have studied displayed activation of several oncogenes (BRAF, RAS, RET/PTC) and inactivation of tumor suppressor genes (TP53) known to be important for thyroid carcinogenesis. Two of the cell lines-TPC1 and FB2-shared the same genotypic profile, probably representing clones of an ancestor cell line (TPC1). Conclusion: Due to their different molecular alterations, these cell lines represent a valuable tool to study the molecular mechanisms underlying thyroid carcinogenesis. We suggest that genotypic analyses should be included as a routine procedure to guarantee the uniqueness of each cell line used in research.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9