Metalaxyl-induced changes in the antioxidant metabolism of Solanum nigrum L. suspension cells

• Tipo: Artigo em Revista Científica Internacional
• Data: 2013

Autores

Alexandra de Sousa (Autor) Outra; Jorge Teixeira (Autor) FCUP; Teresa Regueiras, MT (Autor) Outra; Manuel Azenha (Autor) FCUP; Fernando Silva (Autor) Outra; Fernanda Fidalgo (Autor) FCUP

Revista

Título: Pesticide Biochemistry and Physiology

Vol. 107

Páginas: 235-243

ISSN: 0048-3575

Editora: Elsevier

Indexação

ISI Web of Knowledge - 27 Citações

Scopus - 27 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Medicina básica

Outras Informações

ID Authenticus: P-008-F06

DOI: 10.1016/j.pestbp.2013.07.006

Abstract (EN): The antioxidant responses of Solanum nigrum L. cell suspension cultures to metalaxyl exposure were investigated. An increase in lipid peroxidation and hydrogen peroxide content, for both concentrations tested (20 mg L-1; 40 mg L-1) revealed the response of oxidative metabolism of cell suspensions to metalaxyl. Superoxide dismutase (SOD; EC 1.15.1.1), catalase (CAT; EC 1.11.1.6) and ascorbate peroxidase (APX; EC 1.11.1.11) activities increased, particularly in the highest concentration of metalaxyl used. An analysis by non-denaturing polyacrylamide gel (PAGE) followed by staining for enzyme activity, revealed seven SOD isoenzymes, two CAT isoenzymes, and nine APX isoenzymes. Metalaxyl levels were quantified in the culture medium and results suggest that suspension cells were able to accumulate and/or degrade the fungicide five hours after exposure. SOD, CAT and APX isoenzymes were differently affected by the metalaxyl treatment. Results suggest that the higher concentration of metalaxyl induced oxidative stress to cell suspension cultures of S. nigrum.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9