Murine model of neonatal and infantile pulmonary hypertension induced by monocrotaline: morphometric and hemodynamic evaluation.

• Tipo: Resumo de Comunicação em Conferência Internacional
• Data: 2006

Autores

Henriques-Coelho T (Autor) FMUP; Santos M (Autor) FMUP; Oliveira SM (Autor) FMUP; Gonçalves D (Autor) Outra; Correia-Pinto J (Autor) FMUP; Leite-Moreira AF (Autor) FMUP

Ata de Conferência Internacional

Título: European Heart Journal

Página Inicial: 618

World Congress of Cardiology

Barcelona, SPAIN, 02 a 06 de Setembro de 2006

Classificação Científica

FOS: Ciências médicas e da saúde > Outras ciências médicas

Outras Informações

Resumo (PT): Pulmonary hypertension (PH) is characterized by structural changes of the pulmonary vessels as well as right ventricular (RV) hypertrophy. The reversibility of pulmonary vascular remodeling is possible only at paediatric ages. The monocrotaline (MCT)-induced PH model is well defined for adult animals and mimics human PH. The aim of the study is to establish neonatal and infantile HTP models by MCT administration and to evaluate morphometric and haemodynamic parameters. Protocol 1. Wistar rats 3- and 8-day-old were injected with different doses of MCT: a) 60 mg/kg, n=35; b) 50mg/kg, n=31; c) 30mg/kg, n=40. Protocol 2. Wistar rats with 3 (neonatal) or 8-day-old (infantile) randomly received a subcutaneous injection of MCT 30mg/kg (MCT-Neo, n=10; MCT-Inf, n=10) or an equal volume of vehicle (Ctrl-Neo, n=10; Ctrl-Inf, n=10). At day 21 after injection, RV and left ventricular (LV) peak systolic (RVPmax) and end-diastolic (RVEDP) pressures, peak rates of ventricular pressure rise (dP/dtmax) and relaxation rate (Tau RV) were recorded. At the end, heart and lungs were excised and weighted. Protocol 1. Survival rates at day 21: MCT60=0%; MCT50=0%; MCT30=85%. Protocol 2. Results expressed as means±SE and summarized in table. P<0.05: a vs Ctrl. Protocol 2 - Results Ctrl-Neo MCT-Neo Ctrl-Inf MCT-Inf Body Weight, g 46±1 47±2 80±1 58±3 a RV/LV g/g 0.29±0.02 0.41±0.03 a 0.28±0.02 0.40±0.02 a Lung, g 0.404±0.02 0.478±0.02 0.680±0.28 0.656±0.83 RVPmax, mmHg 18.3±1.9 41.9±1.3 a 19.6±2.8 37.1±4.7 a RVEDP, mmHg 1.1±0.4 2.3±0.5 a 0.4±0.7 2.9±1.4 a dP/dtmax RV, mmHg/s 657±157 1094±106 a 663±74 1210±258 a Tau RV, ms 20±1 31±4 a 21±5 33±9 a We characterized haemodynamics of healthy and PH Wistar rats during the childhood period. We established the survival until 3 weeks after the injection of MCT 30mg/kg. We documented, in both neonatal and infantile groups, prominent RV hypertrophy, pulmonary hypertension and diastolic dysfunction. No differences were found in LV data. This model will allow to study the similarities and differences with the adult model and to test the effects of new drugs in neonatal and infantile PH.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Notas: XVth World Congress of Cardiology, published in journal, European Heart Journal. 2006; Vol.27(Suppl.1):618-618.