1Alpha,25-dihydroxyvitamin D-3 Induces de novo E-cadherin Expression in Triple-negative Breast Cancer Cells by CDH1-promoter Demethylation

• Tipo: Artigo em Revista Científica Internacional
• Data: 2012

Autores

Schmitt F (Adaptador) FMUP

Revista

Título: Anticancer Research

Vol. 32

Páginas: 249-257

ISSN: 0250-7005

Editora: International Institute of Anticancer Research

Indexação

ISI Web of Knowledge - 72 Citações

Scopus - 78 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Outras ciências médicas

Outras Informações

ID Authenticus: P-002-HBE

Abstract (EN): Background: The triple-negative subgroup of breast cancer includes a cluster of tumors exhibiting low E-cadherin expression (metaplastic carcinomas). In several cancer models, lalpha,25-dihydroxyvitamin D-3 (1 alpha,25(OH)(2)D-3) induces differentiation by increasing E-cadherin expression. The Vitamin D receptor (VDR) was evaluated as a possible therapeutic target for metaplastic carcinomas and 1 alpha,25(OH)(2)D-3 effects as a differentiating agent in triple-negative breast cancer cells were assessed. Materials and Methods: Metaplastic carcinomas were assessed for VDR expression by immunohistochemistry; differences in E-cadherin expression in triple-negative breast cancer cells were evaluated by real-time PCR, western blotting and Cadherin 1 (CDH1) methylation status. Results: Most of the metaplastic carcinomas were positive for VDR expression. Furthermore, 1 alpha,25(OH)(2)D-3 promoted differentiation of MDA-MB-231 cells by inducing de novo E-cadherin expression, an effect that was time- and dose-dependent. Also, E-cadherin expression was due to promoter demethylation. Conclusion: Metaplastic carcinomas may respond to 1 alpha,25(OH)(2)D-3, since they express VDR and 1 alpha,25(OH)(2)D-3 induces de novo E-cadherin expression in breast cancer cells by promoter demethylation.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9