Progesterone sensitizes breast cancer MCF7 cells to imatinib inhibitory effects

• Tipo: Artigo em Revista Científica Internacional
• Data: 2008

Autores

rocha, a (Autor) Outra; azevedo, i (Autor) Outra; soares, r (Autor) FMUP

Revista

Título: Journal of Cellular Biochemistry

Vol. 103 Nº 607

Páginas: 607-614

ISSN: 0730-2312

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 7 Citações

Scopus - 9 Citações

Classificação Científica

CORDIS: Ciências da Saúde

Outras Informações

ID Authenticus: P-004-1T2

DOI: 10.1002/jcb.21432

Abstract (EN): In previous Studies, we found that progesterone was able to induce the expression of platelet-derived growth factor (PDGF) in human breast cancer MCF7 cells. Knowing that imatinib rnesylate targets PDGF receptor tyrosine kinase activity, the aim of the present study was to examine the effects of imatinib on progesterone-treated MCF7 cells. Expression of phosphorylated (activated) platelet-derived growth factor receptor-alpha (PDGFR alpha) was detected in MCF7 cells. Interestingly, phosphorylated-PDGFR alpha expression was significantly downregulated by imatinib. The effects of imatinib oil cell growth, apoptosis and migration were then analyzed. Imatinib effectively inhibited anchorage-dependent colony formation, and cell viability as evaluated by MTT assay. Corroborating these findings, a significant increase in the percentage of apoptotic cells was also observed when cells were treated with imatinib. Surprisingly, these inhibitory effects were all enhanced by the presence of progesterone. Cell migration assays did also show a reduction in the migratory capacity after incubation with imatinib. These findings reveal that imatimb acts by decreasing MCF7 cell viability, growth and migration, with concomitant increase in apoptosis. Furthermore, incubation with progesterone seems to prompt cells to the inhibitory action of imatimb, probably by sustaining PDGFRa activity. The Current Study Points Out imatinib as a possible therapeutic strategy in progesterone-dependent breast cancer.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8