An assessment of the clonality of the components of canine mixed mammary tumours by mitochondrial DNA analysis

• Tipo: Artigo em Revista Científica Internacional
• Data: 2009

Autores

Angelica C Bertagnolli (Autor) Outra; Paula Soares (Autor) FMUP; Barbara van Asch (Autor) Outra; Antonio Amorim (Autor) FCUP; Luis Cirnes (Autor) Outra; Valdemar Maximo (Autor) FMUP; Geovanni D Cassali (Autor) Outra

Revista

Título: Veterinary Journal

Vol. 182

Páginas: 269-274

ISSN: 1090-0233

Editora: Elsevier

Indexação

ISI Web of Knowledge - 18 Citações

Scopus - 19 Citações

Classificação Científica

FOS: Ciências agrárias > Ciências veterinárias

Outras Informações

ID Authenticus: P-003-EYM

DOI: 10.1016/j.tvjl.2008.07.005

Abstract (EN): The aim of this study was to investigate if mutations in the mitochondrial DNA (mtDNA) D-loop fragment control region of canine mammary mixed tumours could be used as clonal markers that identified the cell population of origin. Ten benign mixed mammary tumours and nine carcinomas arising from benign mixed tumours were microdissected and DNA from epithelial and mesenchymal tumour cells and from normal mammary tissue was examined for sequence variations in a fragment of the hypervariable control region. Identical sequence variants in both the epithelial and triesenchymal components (as well as in the corresponding normal tissue) were found in 80% of the benign mixed tumours and in 89% of the carcinomas arising from benign mixed tumours suggesting a shared clonal origin. The distinctive sequence alterations identified in the epithelial and mesenchymal components of 15.8%, of all 19 tumours examined, suggests the possibility that a minority of mammary tumours are polyclonal in origin or that early clonal divergence occurs. Increased mutation within the mtDNA D-loop fragment of mixed tumour components was not observed.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6