Expression of LAT1 and LAT2 amino acid transporters in human and rat intestinal epithelial cells

• Tipo: Artigo em Revista Científica Internacional
• Data: 2005

Autores

Sónia Fraga (Autor) FMUP; Maria João Pinho (Autor) FMUP; Patrício Soares da Silva (Autor) FMUP

Revista

Título: Amino Acids

Vol. 29 Nº 3

Páginas: 229-233

ISSN: 0939-4451

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 30 Citações

ISI Web of Science

Scopus - 42 Citações

Pubmed / Medline

Classificação Científica

FOS: Ciências médicas e da saúde > Medicina básica

Outras Informações

ID Authenticus: P-000-0JB

DOI: 10.1007/s00726-005-0221-x

Abstract (EN): The present study evaluated the presence of LAT1 and LAT2 amino acid transporters in human Caco-2 cells and rat IEC-6 cells along the mucosa of the rat digestive tract. The LAT1 cDNA was amplified by PCR using two sets of primers (one specific for rat LAT1 and another simultaneously specific for human, rat and mice). The LAT2 cDNA was amplified by PCR using one set of primers simultaneously specific for human, rat and mice LAT2. The presence of LAT1 and LAT2 protein was examined by means of immunoblotting using an antibody raised against the rat LAT1 and mouse LAT2. Caco-2 and IEC-6 cells, as well as the rat intestinal mucosa, are endowed with both LAT1 and LAT2 transporter transcripts and protein. LAT1 protein is most abundant in IEC-6 cells, which is in agreement with functional data previously reported. The findings in the rat intestinal mucosa indicate that LAT1 protein is most abundant in the colon and its abundance markedly decreases at the level of jejunum and ileum, which contrast with relative homogeneous presence of LAT2 across the digestive tract. In conclusion, Caco-2 and IEC-6 cells, as well as the rat intestinal mucosa, are endowed with both LAT1 and LAT2 amino acid transporter transcripts and protein.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 5