An improved protocol for bacteria identification by MALDI-TOF MS directly from positive blood cultures

• Tipo: Outra Publicação em Revista Científica Internacional
• Data: 2024

Autores

Cruz, S (Autor) Outra; Abreu, D (Autor) Outra; Gomes, R (Autor) Outra; Martins Oliveira, I (Autor) Outra; Silva Dias, A (Autor) Outra; Perez Viso, B (Autor) Outra; Cantón, R (Autor) Outra; pina-vaz, c (Autor) FMUP

Revista

Título: European Journal of Clinical Microbiology and Infectious Diseases

ISSN: 0934-9723

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 1 Citação

Scopus - 1 Citação

Outras Informações

ID Authenticus: P-00Z-KJQ

DOI: 10.1007/s10096-023-04725-3

Abstract (EN): FASTinov (R) developed a rapid antimicrobial susceptibility test that includes the purification of a bacterial suspension directly from positive blood cultures (BC). In order to streamline laboratory workflow, the use of the bacterial suspension obtained through FASTinov (R) sample prep was tested for identification (ID) by matrix absorption laser deionization-time of flight mass spectrometry (MALDI-TOF MS) (Bruker) in 364 positive BC, and its accuracy assessed comparing with the MALDI-TOF MS ID of the next-day subcultured colonies. FASTinov sample prep was highly reliable for rapid ID directly from BC with proportion of agreement of 94.9% for Gram-positive and 96.3% for Gram-negative bacteria.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6