In situ functionalization of wet-spun fibre meshes for bone tissue engineering

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

Leonor, IB (Autor) Outra; Rodrigues, GT (Autor) Outra; Gomes, ME (Autor) Outra; Rui Reis (Autor) Outra

Revista

Título: Journal of tissue engineering and regenerative medicine

Vol. 5

Páginas: 104-111

ISSN: 1932-6254

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 33 Citações

Scopus - 38 Citações

Outras Informações

ID Authenticus: P-002-VNT

DOI: 10.1002/term.294

Abstract (EN): Bone tissue engineering success strongly depends on our ability to develop new materials combining osteoconductive, osteoinductive and osteogenic properties. Recent studies suggest that biomaterials incorporating silanol (Si-OH) groups promote and maintain osteogenesis. The purpose of the present research work was to provide evidence that using wet-spinning technologies and a calcium silicate solution as a coagulation bath, it was possible to develop an in situ functionalization methodology to obtain 3D wet-spun fibre meshes with Si-OH groups, through a simple, economic and reliable process. SPCL (blend of starch with polycaprolactone) fibre meshes were produced by wet-spinning, using a calcium silicate solution as a non-solvent and functionalized in situ with Si-OH groups. In vitro tests, using goat bone marrow stromal cells (GBMSCs), showed that SPCL-Si scaffolds sustained cell viability and proliferation. Furthermore, high ALP activity and matrix production indicated that Si-OH groups improve cellular functionality towards the osteoblastic phenotype. Using this methodology, and assembling several wet-spun fibre meshes, 3D meshes can be developed, aiming at designing osteoconductive/osteoinductive 3D structures capable of stimulating bone ingrowth in vivo. Copyright (C) 2010 John Wiley & Sons, Ltd.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8