Bilayered constructs aimed at osteochondral strategies: The influence of medium supplements in the osteogenic and chondrogenic differentiation of amniotic fluid-derived stem cells

• Tipo: Artigo em Revista Científica Internacional
• Data: 2012

Autores

Rodrigues, MT (Autor) Outra; Lee, SJ (Autor) Outra; Gomes, ME (Autor) Outra; Rui Reis (Autor) Outra; Atala, A (Autor) Outra; Yoo, JJ (Autor) Outra

Revista

Título: Acta Biomaterialia

Vol. 8 Nº 3

Páginas: 2795-2806

ISSN: 1742-7061

Editora: Elsevier

Indexação

ISI Web of Knowledge - 45 Citações

Scopus - 56 Citações

Outras Informações

ID Authenticus: P-002-8BF

DOI: 10.1016/j.actbio.2012.04.013

Abstract (EN): The development of osteochondral tissue engineered interfaces would be a novel treatment for traumatic injuries and aging associated diseases that affect joints. This study reports the development of a bilayered scaffold, which consists of both bone and cartilage regions. On the other hand, amniotic fluid-derived stem cells (AFSCs) could be differentiated into either osteogenic or chondrogenic cells, respectively. In this study we have developed a bilayered scaffolding system, which includes a starch/polycaprolactone (SPCL) scaffold for osteogenesis and an agarose hydrogel for chondrogenesis. AFSC-seeded scaffolds were cultured for 1 or 2 weeks in an osteochondral-defined culture medium containing both osteogenic and chondrogenic differentiation factors. Additionally, the effect of the presence or absence of insulin-like growth factor-1 (IGF-1) in the culture medium was assessed. Cell viability and phenotypic expression were assessed within the constructs in order to determine the influence of the osteochondral differentiation medium. The results indicated that, after osteogenic differentiation, AFSCs that had been seeded onto SPCL scaffolds did not require osteochondral medium to maintain their phenotype, and they produced a protein-rich, mineralized extracellular matrix (ECM) for up to 2 weeks. However, AFSCs differentiated into chondrocyte-like cells appeared to require osteochondral medium, but not IGF-1, to synthesize ECM proteins and maintain the chondrogenic phenotype. Thus, although IGF-1 was not essential for creating osteochondral constructs with AFSCs in this study, the osteochondral supplements used appear to be important to generate cartilage in long-term tissue engineering approaches for osteochondral interfaces. In addition, constructs generated from agarose-SPCL bilayered scaffolds containing pre-differentiated AFSCs may be useful for potential applications in regeneration strategies for damaged or diseased joints.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 12