Encapsulation of adipose-derived stem cells and transforming growth factor-beta 1 in carrageenan-based hydrogels for cartilage tissue engineering

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

Rocha, PM (Autor) Outra; Santo, VE (Autor) Outra; Gomes, ME (Autor) Outra; Rui Reis (Autor) Outra; Mano, JF (Autor) Outra

Revista

Título: Journal of Bioactive and Compatible Polymers

Vol. 26

Páginas: 493-507

ISSN: 0883-9115

Editora: SAGE

Indexação

ISI Web of Knowledge - 65 Citações

Scopus - 74 Citações

Outras Informações

ID Authenticus: P-002-N40

DOI: 10.1177/0883911511420700

Abstract (EN): Tissue engineering (TE) is an emerging field for the regeneration of damaged tissues. The combination of hydrogels with stem cells and growth factors (GFs) has become a promising approach to promote cartilage regeneration. In this study, carrageenan-based hydrogels were used to encapsulate both cells and transforming growth factor-beta 1 (TGF-beta 1). The ATDC5 cell line was encapsulated to determine the cytotoxicity and the influence of polymer concentration on cell viability and proliferation. Human adipose-derived stem cells (hASCs) were encapsulated with TGF-beta 1 in the hydrogel networks to enhance the chondrogenic differentiation of hASCs. Specific cartilage extracellular matrix molecules expression by hASCs were observed after 14 days of cultures of the constructs under different conditions. The kappa-carrageenan was found to be a suitable biomaterial for cell and GF encapsulation. The incorporation of TGF-beta 1 within the carrageenan-based hydrogel enhanced the cartilage differentiation of hASCs. These findings indicate that this new system for cartilage TE is very promising for injectable thermoresponsive formulation applications.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 15