LexA, a transcription regulator binding in the promoter region of the bidirectional hydrogenase in the cyanobacterium Synechocystis sp PCC 6803

• Tipo: Artigo em Revista Científica Internacional
• Data: 2005

Autores

Oliveira, P (Autor) Outra; Lindblad, P (Autor) Outra

Revista

Título: FEMS Microbiology Letters

Vol. 251

Páginas: 59-66

ISSN: 0378-1097

Editora: Oxford University Press

Indexação

ISI Web of Knowledge - 40 Citações

Scopus - 60 Citações

Outras Informações

ID Authenticus: P-00F-RG8

DOI: 10.1016/j.femsle.2005.07.024

Abstract (EN): The unicellular cyanobacterium Synechocystis PCC 6803 contains a single pentameric bidirectional hydrogenase encoded by hox-EFUYH. Transcriptional experiments demonstrated that the five hox genes are part of a single transcript together with three ORFs with unknown functions. The transcription start point was localized by 5' RACE to 168bp upstream the hoxE ATG start codon. DNA affinity assays demonstrated a specific interaction between the hox regulatory promoter region and a protein which, using mass spectrometry, was identified to be LexA. Overexpressed His-tagged Synechocystis LexA and EMSA showed a specific binding to the promoter region of the hox operon. Increasing concentrations of the purified LexA resulted in two retarded LexA-DNA complexes, in agreement with the presence of two putative LexA binding sites upstream the determined TSP.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8