An improved method for high-quality RNA isolation from needles of adult maritime pine trees

• Tipo: Artigo em Revista Científica Internacional
• Data: 2003

Autores

Azevedo, H (Autor) Outra; Lino Neto, T (Autor) Outra; Tavares, RM (Autor) Outra

Revista

Título: Plant Molecular Biology Reporter

Vol. 21

Páginas: 333-338

ISSN: 0735-9640

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 69 Citações

Scopus - 75 Citações

Outras Informações

ID Authenticus: P-000-EC7

DOI: 10.1007/bf02772582

Abstract (EN): When conventional RNA isolation methods optimized for pine seedlings are applied to needles of adult pine trees, poor-quality RNA results. Here we describe a modified procedure to isolate high-quality RNA from needles of 30-year-old maritime pines, exhibiting high levels of phenolics, polysaccharides, and RNases. Major changes are the inclusion of proteinase K in the extraction medium followed by incubation at 42degreesC. Integrity and purity were evaluated by using denaturing gel electrophoresis and spectrophotometry (A(260)/A(230) and A(260)/A(280)). The total RNA Could be successfully used for poly(A)(+)-RNA isolation and cDNA library construction.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6