Analysis on the Role of Phenylpropanoid Metabolism in the Pinus pinaster-Botrytis cinerea Interaction

• Tipo: Artigo em Revista Científica Internacional
• Data: 2010

Autores

Azevedo, H (Autor) Outra; Dias, A (Autor) Outra; Tavares, RM (Autor) Outra

Revista

A Revista está pendente de validação pelos Serviços Administrativos.

Título: JOURNAL OF PHYTOPATHOLOGY

Vol. 158

Páginas: 641-646

ISSN: 0931-1785

Indexação

ISI Web of Knowledge - 6 Citações

Scopus - 6 Citações

Outras Informações

ID Authenticus: P-003-3N6

DOI: 10.1111/j.1439-0434.2010.01673.x

Abstract (EN): The interaction between maritime pine (Pinus pinaster) and the necrotrophic pathogen Botrytis cinerea was addressed at the level of phenylpropanoid metabolism using a suspension cell model system. HPLC-DAD analysis revealed the presence of several phenolic compounds, including derivatives of epicatechin, caffeoylquinic acid and glycosylated quercetin. However, challenged cells evidenced a reduction in total soluble phenolics and a decrease in the lignin content of cells. Key phenylpropanoid metabolism genes Pal and Chs were isolated after screening a P. pinaster cDNA library. Expression analysis evidenced a downregulation of Pal transcripts. Chs transcripts were observed in P. pinaster needles but not in suspension cells. With regard to the P. pinaster-B. cinerea interaction, results indicate that elicited cells downregulate phenylpropanoid metabolism, which suggests that systemic acquired resistance is not induced after challenging with this necrotrophic pathogen.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6