The effect of hyaluronan-based delivery of stromal cell-derived factor-1 on the recruitment of MSCs in degenerating intervertebral discs

• Tipo: Artigo em Revista Científica Internacional
• Data: 2014

Autores

Pereira, CL (Autor) Outra; Goncalves, RM (Autor) ICBAS; Peroglio, M (Autor) Outra; Pattappa, G (Autor) Outra; D'Este, M (Autor) Outra; Eglin, D (Autor) Outra; Barbosa, MA (Autor) Outra; Alini, M (Autor) Outra; Grad, S (Autor) Outra

Revista

Título: Biomaterials

Vol. 35

Páginas: 8144-8153

ISSN: 0142-9612

Editora: Elsevier

Indexação

ISI Web of Knowledge - 68 Citações

Scopus - 83 Citações

Outras Informações

ID Authenticus: P-009-NXP

DOI: 10.1016/j.biomaterials.2014.06.017

Abstract (EN): Intervertebral disc (IVD) degeneration is the leading cause of low back pain and disability in the active population. Transplantation of mesenchymal stem cells (MSCs) in a hydrogel carrier can induce regenerative effects in degenerated IVDs. Moreover, it was found that degenerative discs release chemo-attractants effective in MSC recruitment. Based on these findings, we hypothesized that an injectable hydrogel that can enhance the number of migrated MSCs in the IVD and provide a suitable matrix for their survival and differentiation would be ideal. The purpose of this study was to evaluate the potential of a thermoreversible hyaluronan-poly(N-isopropylacrylamide) (HAP) hydrogel as chemoattractant delivery system to recruit human MSCs in degenerative IVDs. The results demonstrate that HAP hydrogels containing stromal cell derived factor-1 (SDF-1) significantly increased the number of MSCs migrating into nucleotomized discs compared with discs treated with only HAP or SDF-1 in solution. HAP hydrogels releasing SDF-1 enhanced both the number of recruited cells and their migration distance in the IVD tissue. Furthermore, this phenomenon was dependent on MSC donor age. In conclusion, HAP SDF-1 is effective for the recruitment of stem cells in the IVD, thus opening new possibilities for the development of regenerative therapies based on endogenous cell migration.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10