Poly(gamma-glutamic acid) and poly(gamma-glutamic acid)-based nanocomplexes enhance type II collagen production in intervertebral disc

• Tipo: Artigo em Revista Científica Internacional
• Data: 2017

Autores

Antunes, JC (Autor) Outra; Pereira, CL (Autor) Outra; Teixeira, GQ (Autor) Outra; Silva, RV (Autor) Outra; Caldeira, J (Autor) Outra; Grad, S (Autor) Outra; Goncalves, RM (Autor) ICBAS; Barbosa, MA (Autor) Outra

Revista

Título: Journal of Materials Science: Materials in Medicine

Vol. 28

ISSN: 0957-4530

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 19 Citações

Scopus - 21 Citações

Outras Informações

ID Authenticus: P-00M-81Z

DOI: 10.1007/s10856-016-5787-1

Abstract (EN): Intervertebral disc (IVD) degeneration often leads to low back pain, which is one of the major causes of disability worldwide, affecting more than 80% of the population. Although available treatments for degenerated IVD decrease symptoms' progression, they fail to address the underlying causes and to restore native IVD properties. Poly(gamma-glutamic acid) (gamma-PGA) has recently been shown to support the production of chondrogenic matrix by mesenchymal stem/stromal cells. gamma-PGA/chitosan (Ch) nanocomplexes (NCs) have been proposed for several biomedical applications, showing advantages compared with either polymer alone. Hence, this study explores the potential of gamma-PGA and gamma-PGA/Ch NCs for IVD regeneration. Nucleotomised bovine IVDs were cultured ex vivo upon injection of gamma-PGA (pH 7.4) and gamma-PGA/Ch NCs (pH 5.0 and pH 7.4). Tissue metabolic activity and nucleus pulposus DNA content were significantly reduced when NCs were injected in acidic-buffered solution (pH 5.0). However, at pH 7.4, both gamma-PGA and NCs promoted sulphated glycosaminoglycan production and significant type II collagen synthesis, as determined at the protein level. This study is a first proof of concept that gamma-PGA and gamma-PGA/Ch NCs promote recovery of IVD native matrix, opening new perspectives on the development of alternative therapeutic approaches for IVD degeneration.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 17