MESENCHYMAL STEM CELL SECRETOME DECREASES THE INFLAMMATORY RESPONSE IN ANNULUS FIBROSUS ORGAN CULTURES

• Tipo: Artigo em Revista Científica Internacional
• Data: 2021

Autores

Neidlinger Wilke, C (Autor) Outra; Ekkerlein, A (Autor) Outra; Goncalves, RM (Autor) ICBAS; Ferreira, JR (Autor) Outra; Ignatius, A (Autor) Outra; Wilke, HJ (Autor) Outra; Teixeira, GQ (Autor) Outra

Revista

Título: European Cells and Materials

Vol. 42

Páginas: 1-19

ISSN: 1473-2262

Editora: AO Research Institute Davos

Indexação

ISI Web of Knowledge - 7 Citações

Scopus - 9 Citações

Outras Informações

ID Authenticus: P-00V-981

DOI: 10.22203/ecm.v042a01

Abstract (EN): Mesenchymal stem/stromal cell (MSC)-based therapies have been proposed for back pain and discdegeneration, despite limited knowledge on their mechanism of action. The impact of MSCs/their secretomeon annulus fibrosus (AF) cells and tissue was analysed in bovine AF organ cultures (AF-OCs) exposed toupper-physiological cyclic tensile strain (CTS, 9 %, 1 Hz, 3 h/d) and interleukin (IL)-1 beta in a custom-made device. A 4 d treatment of the CTS + IL-1 beta-stimulated AF-OCs with MSC secretome downregulated the expression of inflammation markers [IL-6, IL-8, prostaglandin-endoperoxide synthase 2 (PTGS2)], complement system regulators [cluster of differentiation (CD)46, CD55, CD59] and matrix metalloproteinase 1 but alsoof tissue inhibitors of metalloproteinases (TIMP-1, TIMP-2) and collagen type I. At the protein level, it was confirmed that IL-6, MMP-3 and collagen content was decreased in AF-OCs treated with the MSC secretome compared to the CTS + IL-1 beta stimulation alone. 9 d after treatment, a biomechanical peel-force test showed that the annular adhesive strength was significantly decreased by the MSC secretome treatment. Overall, MSC secretome had a stronger impact on AF tissue than MSCs in co-culture. The secretome contributed to a decrease in the inflammatory and catabolic status of AF cells activated by CTS + IL-1 beta and played a role in the regulation of the complement system. However, it also contributed to a decrease in collagen at the gene/protein level and in AF mechanical strength compared to the CTS + IL-1 beta stimulation alone. Therefore, the use of MSC secretome requires further investigation regarding its influence on disc matrix properties.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 19